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Target capture and whole genome sequencing for Lepidoptera phylogenomics: Museum specimens and beyond
Presented by: Marianne Espeland, Leibniz Institute for the Analysis of Biodiversity Change, Zoological Research Museum Alexander Koenig, Bonn, Germany
Abstract: Reduced representation techniques like target capture have been popular for phylogenomic studies for several years, both for fresh and old specimens. Such methods only sequence predefined genomic regions (exons, UCEs etc.) and are cost-efficient. They are also attractive for museomics since the coverage for each sequenced region on average is higher, than when sequencing the entire genome. Due to reduced sequencing costs large-scale whole genome sequencing (WGS) is now feasible for most Lepidoptera species. In this talk I will present our work on phylogenomics and museomics of multiple Lepidoptera groups using both of these methodologies. I will give multiple examples from species delimitation to higher-level phylogenomics, discuss the pros and cons of both methods and what we have learned so far, including some (hopefully) good advice for labwork and bioinformatic processing.
Watch on ZoomWatch previously recorded webinars here
Thomas C. Emmel Seminar Series presents: Expanding Horizons in Lepidoptera Research
Continuing from Fall 2020, the McGuire Center is hosting this webinar series as an opportunity for both early career researchers as well as established leaders in the field to present their work. We hope that you will join us to hear about current advances in many diverse fields of Lepidoptera research.